Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes

Authors
Cosa, G Focsaneanu, KS McLean, JRN Scaiano, JC
Citation
G. Cosa et al., Direct determination of single-to-double stranded DNA ratio in solution applying time-resolved fluorescence measurements of dye-DNA complexes, CHEM COMMUN, (8), 2000, pp. 689-690
Citations number
17
Categorie Soggetti
Chemistry
Journal title
CHEMICAL COMMUNICATIONS
ISSN journal
13597345 → ACNP
Issue
8
Year of publication
2000
Pages
689 - 690
Database
ISI
SICI code
1359-7345(2000):8<689:DDOSSD>2.0.ZU;2-2
Abstract
We report the fluorescence lifetimes of the DNA-stain dye PicoGreen and dis cuss the difference exhibited in the upon binding to single-stranded vs. do uble-stranded DNA; we here developed a direct method for determining single -to-double stranded DNA ratios in solution by measurement of the pre-expone ntial factors in the fluorescence decay traces of dye-DNA complexes.