Production of viral vectors for gene therapy applications

Advances in cell culture engineering, cell metabolism, bioreactor design an d operation, and downstream processing will all positively impact the biopr ocessing of viral vectors. Design of appropriate vectors and tailoring of p ackaging cells to support more productive infections will be of paramount i mportance for production of high-titer and high-quality vectors. Furthermor e, quantitative analysis of the infection parameters during virus propagati on, such as time of infection, multiplicity of infection, the length of rep lication cycle, virus half-life, and burst size, will also be important to the process optimization. Finally, procedures for separation, purification and formulation of vector preparations have to be further developed.