Physical linkage of the lysyl oxidase-like (LOXL1)gene to the PML gene on human chromosome 15q22

A contig was constructed centered on the PML (promyelocytic leukemia) gene. Using an exon-trapping approach to identify potential genes from a pool of cosmids located 5' of the PML gene, four exons were identified that showed 100% sequence homology with the previously cloned lysyl oxydase-like (LOXL 1) gene. An exon probe identified a single transcript of 2.4 kb on a multit issue Northern blot with a pattern identical to the one reported for the LO XL1 gene. Pulsed-field gel electrophoresis showed comigrating bands for bot h the PML cDNA and LOXL1 probes, demonstrating a physical linkage between t hese two genes. These data provide physical mapping information to compleme nt the previous cytogenetic localization of LOXL1. Copyright (C) 2000 S. Ka rger AG, Basel.