A contig was constructed centered on the PML (promyelocytic leukemia) gene.
Using an exon-trapping approach to identify potential genes from a pool of
cosmids located 5' of the PML gene, four exons were identified that showed
100% sequence homology with the previously cloned lysyl oxydase-like (LOXL
1) gene. An exon probe identified a single transcript of 2.4 kb on a multit
issue Northern blot with a pattern identical to the one reported for the LO
XL1 gene. Pulsed-field gel electrophoresis showed comigrating bands for bot
h the PML cDNA and LOXL1 probes, demonstrating a physical linkage between t
hese two genes. These data provide physical mapping information to compleme
nt the previous cytogenetic localization of LOXL1. Copyright (C) 2000 S. Ka
rger AG, Basel.