Cloning and expression of CIS6, chromosome assignment to 3p22 and 2p21 by in situ hybridization

A family of negative regulators of JAK signaling pathway referred to as sup pressor of cytokines signaling (SOCS) or cytokine-inducible SH2 protein (CI S) has been recently identified. In order to find additional members of thi s family, we have used a consensus amino acid sequence contained in the wel l-conserved central SH2 domain to search DNA. databases. We isolated cDNA c oding for the human homologue of SOCS-5, referred to as CIS6. Northern blot analysis revealed CIS6 mRNA expression in various tissues such as heart, m uscle, spleen, and thymus and in all myeloma cell lines examined. The gene was assigned to human chromosome bands 2p21 and 3p22 by in situ hybridizati on. CIS6 is structurally related to other members of the CIS family and the refore could act as a negative regulator of signal transduction. Copyright (C) 2000 S. Karger AG, Basel.