DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells

A novel DNA polymerase has been identified in human cells, Human DNA polyme rase mu (Pol mu), consisting of 494 amino acids, has 41% identity to termin al deoxynucleotidyltransferase (TdT), Human Pol mu, overproduced in Escheri chia coli in a soluble form and purified to homogeneity, displays intrinsic terminal deoxynucleotidyltransferase activity and a strong preference for activating Mn2+ ions. Interestingly, unlike TdT, the catalytic efficiency o f polymerization carried out by Pol mu was enhanced by the presence of a te mplate strand. Using activating Mg2+ ions, template-enhanced polymerization was also template-directed, leading to the preferred insertion of compleme ntary nucleotides, although with low discrimination values. In the presence of Mn2+ ions, template-enhanced polymerization produced a random insertion of nucleotides. Northern-blotting and in situ analysis showed a preferenti al expression of Pol mu mRNA in peripheral lymphoid tissues. Moreover, a la rge proportion of the human expressed sequence tags corresponding to Pol mu , present in the databases, derived from germinal center B cells. Therefore , Pol mu is a good candidate to be the mutator polymerase responsible for s omatic hypermutation of immunoglobulin genes.