Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin

Citation
M. Wentworth et al., Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin, FEBS LETTER, 471(1), 2000, pp. 71-74
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
471
Issue
1
Year of publication
2000
Pages
71 - 74
Database
ISI
SICI code
0014-5793(20000407)471:1<71:CFQIIL>2.0.ZU;2-T
Abstract
Non-photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xan thophyll cycle. A nem in vitro model for this process has been developed. P urified light harvesting complexes above the detergent critical micelle con centration have a stable high fluorescence yield but a rapidly inducible fl uorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was w ithout effect, lutein and antheraxanthin induced a marginal response, where as the violaxanthin analogue, auroxanthin, induced strong quenching. Quench ing was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin-dependent alter ation in the chlorophyll environment. (C) 2000 Federation of European Bioch emical Societies.