In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats

Authors
Shiota, G Kunisada, T Oyama, K Udagawa, A Nomi, T Tanaka, K Tsutsumi, A Isono, M Nakamura, T Hamada, H Sakatani, T Sell, S Sato, K Ito, H Kawasaki, H
Citation
G. Shiota et al., In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats, FEBS LETTER, 470(3), 2000, pp. 325-330
Citations number
32
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
470
Issue
3
Year of publication
2000
Pages
325 - 330
Database
ISI
SICI code
0014-5793(20000331)470:3<325:IVTOHG>2.0.ZU;2-B
Abstract
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, rye transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats mere infected with a recombinant adenoviru s carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased a t each time point. Our results suggest that in vivo transfer of the HGF gen e into liver accelerates proliferation of hepatic oval cells in the Solt-Fa rber model in rats. (C) 2000 Federation of European Biochemical Societies.