Analysis of Leptospira isolates from mainland Portugal and the Azores islands

Citation
M. Collares-pereira et al., Analysis of Leptospira isolates from mainland Portugal and the Azores islands, FEMS MICROB, 185(2), 2000, pp. 181-187
Citations number
20
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
185
Issue
2
Year of publication
2000
Pages
181 - 187
Database
ISI
SICI code
0378-1097(20000415)185:2<181:AOLIFM>2.0.ZU;2-N
Abstract
From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibod ies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic technique s, a sample of isolates was analyzed through pulsed-field agarose gel elect rophoresis (PFGE) (n = 71), mapped restriction site polymorphisms (MRSPs) i n PCR-amplified rRNA genes (n = 45, including 13 saprophytes) and arbitrari ly primed polymerase chain reaction (AP-PCR) fingerprinting (n = 32). MRSP and AP-PCR lead to species identification of the studied 32 pathogenic isol ates: Leptospira interrogans (n = 3), Leptospira borgpetersenii (n = 8) and Leptospira bougpetersenii (n = 21). MAbs and PFGE characterized pathogenic isolates at the serovar level and resulted mainly in agreement (64%) altho ugh many discrepancies (35%) were observed. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights r eserved.