Isolation and quantification of triterpenoid acids from Ganoderma applanatum of Istrian origin

Various Ganoderma spp. have been known for their wide range of pharmacologi cally active compounds. From Ganoderma applanatum of Istrian origin, growin g on Populus, triterpenoid acids were isolated and quantified. An in-house relational information system was built and used as a support for isolation and identification of triterpenoids from different layers of G. applanatum fruiting bodies. An optimised extraction procedure was developed, and IR, UV and NMR spectra were used for the identification of ganoderic and ganode renic acids. The highest fraction of triterpenoid acids was found in the tu bes (6.4 mg/g of air-dry weight), followed by the dark context layer, which is the young part of the pileus (2.5 mg/g). The white context layer of the older pileus and the upper surface of the fruiting body contained only abo ut 0.6 mg/g of triterpenoid acids.