Prr. Gangula et al., Pregnancy and sex steroid hormones enhance circulating calcitonin gene-related peptide concentrations in rats, HUM REPR, 15(4), 2000, pp. 949-953
Calcitonin-gene-related peptide (CGRP) is a 37 amino acid neuropeptide synt
hesized primarily in dorsal root ganglia (DRG) and distributed widely in th
e perivascular nerves, suggesting that this peptide may play a role in the
regulation of peripheral vascular tone, Since female sex steroid hormones h
ave been implicated in the regulation of peripheral vascular tone during pr
egnancy, we postulated that they may alter the concentration of CGRP in the
circulation and thus modulate the increased blood flow observed during pre
gnancy. In the present study, we measured changes in plasma concentrations
of CGRP in non-pregnant, pregnant, and post-partum rats. Groups of ovariect
omized rats were treated s.c. for 3 days either with 17 beta-oestradiol (2.
5 mu g per injection twice daily), progesterone (2 mg per injection twice d
aily), or vehicle. Another group of adult, non-pregnant rats at dioestrus s
tage of the oestrous cycle was also used in this study, Plasma concentratio
ns of CGRP were higher (P < 0.05) in rats on day 19 of pregnancy (22.0 +/-
3.0 pmol/l) compared to that during delivery(5.0 +/- 2.0), post-partum day
2 (2.0 +/- 0.7) or in non-pregnant (4.9 +/- 1.6) state. Furthermore, in adu
lt ovariectomized (6.0 +/- 0.6) rats, plasma CGRP concentrations were incre
ased significantly (P < 0.05) by oestradiol (10.0 +/- 1.0), progesterone (9
.5 +/- 1.0) and oestradiol + progesterone (14.0 +/- 1.0). Thus, circulating
concentrations of CGRP are elevated during pregnancy and by oestrogen and
progesterone, suggesting that the elevated concentrations of CGRP may play
an important role in vascular adaptations that occur during pregnancy.