P. Harel et al., Mechanical properties of sugar beet Ca-pectate gel usable for cell immobilisation and heavy metal accumulation, IND CROP PR, 11(2-3), 2000, pp. 259-264
After alkaline hydrolysis, pectin extracted from sugar beet pulp is able to
form gels that can be used for whole cell entrapment and heavy metal accum
ulation. The purpose of the present work was to appraise the ability of sug
ar beet pectin (SBP) gel particles to accommodate the stresses encountered
in bioreactors. We investigated the rheological properties (mechanical beha
vior) of SBP gels shaped as flat membranes and compared them to those of ci
trus pectin and algal alginate gel membranes. We evaluated the fracture pro
perties of the different gel membranes by constructing stress-strain curves
(obtained by uniaxial compression at constant speed). Stress and strain at
fracture, Young's modulus, fracture and flow energies were determined. SBP
and citrus pectin gel membranes were less resistant to stress than algal a
lginate membranes, with stresses at fracture of 1.7 x 10(5) N m(-2)(SBP) an
d 4.83 x 10(5) N m(-2) (citrus pectin) as compared to 2.44 x 10(6) N m(-2)
for alginate. A chain flow phenomenon prior to fracture was observed in the
case of pectin gel membranes (flow energies: 3500 J m(-3) for SBP; 4400 J
m(-3) for citrus pectin, negligible for alginate). These results show that
pectin gels are more heterogeneous than those of algal alginate and behave
as a viscoelastic material, in opposition with alginate gels that display a
n elastic behaviour. The relatively low mechanical resistance of SBP gels m
ay impede their long-term utilization in the biological treatment of wastes
. (C) 2000 Elsevier Science B.V. All rights reserved.