Polyclonal antibodies marking the radial nerve cells in the starfish Asterias amurensis

Citation
Mg. Eliseikina et al., Polyclonal antibodies marking the radial nerve cells in the starfish Asterias amurensis, IAN BIOL, (2), 2000, pp. 149-152
Citations number
27
Categorie Soggetti
Biology
Journal title
IZVESTIYA AKADEMII NAUK SERIYA BIOLOGICHESKAYA
ISSN journal
00023329 → ACNP
Issue
2
Year of publication
2000
Pages
149 - 152
Database
ISI
SICI code
0002-3329(200003/04):2<149:PAMTRN>2.0.ZU;2-G
Abstract
The results of preliminary studies suggest that the cytoskeletal fraction o f the radial nerve of the starfish Asterias amurensis contained a 32 kDa pr otein, which is tissue specific, This protein was isolated from the radial nerve by preparative electrophoresis and used as an antigen for raising pol yclonal antibodies. When testing these antibodies on sections of the starfi sh tissues, it was shown that they interact only with the proteins, present in the radial nerve cells. A conclusion was drawn that the raised antibodi es may be used as a cell marker when studying regeneration of the nervous s ystem in starfish.