Kinetics of active sucrose transport in Saccharomyces cerevisiae

Authors
Stambuk, BU Batista, AS De Araujo, PS
Citation
Bu. Stambuk et al., Kinetics of active sucrose transport in Saccharomyces cerevisiae, J BIOSCI BI, 89(2), 2000, pp. 212-214
Citations number
25
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
13891723 → ACNP
Volume
89
Issue
2
Year of publication
2000
Pages
212 - 214
Database
ISI
SICI code
1389-1723(200002)89:2<212:KOASTI>2.0.ZU;2-F
Abstract
The kinetic analysis of active sucrose-H+ uptake by Saccharomyces cerevisia e revealed the presence of two transport systems with high and low affinity for sucrose. The MAL2T permease has a low affinity (K-m=120+/-20 mM) for s ucrose, while the alpha-glucoside transporter encoded by the AGT1 gene is a high affinity sucrose-H+ symporter (K-m=7.9+/-0.8 mM) that increases the s pecific growth rate of cells growing on sucrose.