Mg. Stevens et al., COMPARATIVE EFFECTS OF BOVINE CYTOKINES ON CATTLE AND BISON PERIPHERAL-BLOOD MONONUCLEAR CELL-PROLIFERATION, Comparative immunology, microbiology and infectious diseases, 20(2), 1997, pp. 155-162
Proliferation of peripheral blood mononuclear cells (PBMC) from cattle
and bison was measured following stimulation of PBMC with bovine cyto
kines. Bovine interleukin 1 beta (BoIL-1 beta), interteukin 2 (BoIL-2)
or granulocyte-macrophage colony-stimulating factor (BoGM-CSF) at 0.1
-100 U/ml were incubated for 48 h with PBMC alone or with PBMC and var
ious mitogens. These included concanavalin A (Con A), phytohemagglutin
in (PHA), pokeweed mitogen (PWM) or Escherichia coli 055:B5 lipopolysa
ccharide (IFS) at 10-0.1 mu g/ml. BoIL-2 alone, but not BoIL-1 beta an
d BoGM-CSF alone, induced proliferation of cattle and bison PBMC in th
e absence of mitogens. In addition, BoIL-1 beta and BoIL-2. but not Bo
GM-CSF, enhanced proliferation of cattle and bison PBMC induced by mit
ogens. These results indicate that BoIL-1 beta and BoIL-2 stimulate ca
ttle and bison PBMC proliferation in a similar manner, whereas BoGM-CS
F does not appear capable of stimulating either cattle or bison PBMC p
roliferation.