FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy

Citation
T. Takizawa et Jm. Robinson, FluoroNanogold is a bifunctional immunoprobe for correlative fluorescence and electron microscopy, J HIST CYTO, 48(4), 2000, pp. 481-485
Citations number
14
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
ISSN journal
00221554 → ACNP
Volume
48
Issue
4
Year of publication
2000
Pages
481 - 485
Database
ISI
SICI code
0022-1554(200004)48:4<481:FIABIF>2.0.ZU;2-A
Abstract
We applied a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to immunocytochemistry on ultrathin cryosections. FNG has the properties o f both a fluorescent dye-conjugated antibody for fluorescence microscopy an d a gold particle-conjugated antibody for electron microscopy. Therefore, t his bifunctional immunoprobe permits correlative microscopic observation of the same cell profiles labeled in a single labeling procedure by these two imaging methods. We demonstrate the utility of FNG as a secondary antibody for immunocytochemical labeling of myeloperoxidase (a marker protein for a zurophilic granules) in ultrathin cryosectioned human neutrophils. Its dete ction requires high spatial resolution because neutrophils contain many cyt oplasmic granules. There was a one-to-one relationship between fluorescent structures labeled with FNG and organelle profiles labeled with the same si lver-enhanced FNG in ultrathin cryosections. Use of FNG immunocytochemistry on ultrathin cryosections is an ideal methodology for high-resolution corr elative fluorescence and electron microscopy and can provide unique informa tion that may be difficult to obtain with a single imaging regimen.