The separation of oxybutynin enantiomers (racemic mixture of two enantiomer
s) was studied on an ovomucoid column. The optimized chiral separation base
line resolved the enantiomers in less than 10 minutes. Chromatographic vari
ables that were found to have an effect on the chiral separation were studi
ed and include: mobile phase pH, mobile phase ionic strength, type and conc
entration of organic modifier added to the mobile phase, column temperature
, and the amount of analyte injected.
The enantiomeric separation was optimized on the ovomucoid column based on
how each variable affected the separation. Calibration curves for a standar
d were linear over a range of 8.36 to 668.8 mu g/g (ppm) with a correlation
coefficient of 0.999 for both enantiomers. A detection limit of 4.5 mu g/g
and a quantitation limit of 9.0 mu g/g were also found.