Regeneration of plantlets from leaf explant of groundnut, Arachis hypogaeaL.

Direct shoot regeneration was induced from leaf explants of Arachis, grown in vitro. Shoot buds appeared at the cut surface of the vein within 8-15 da ys of culture and developed into shoots within 25-30 days. Among the differ ent concentrations of BAP used (1 to 6 mg/l), MS medium supplemented with 4 mg/l BAP along with 0.5 mg/l IAA gave high frequency of shoot bud initiati on (62.19%). Gradual increase in shoot induction (32 to 62%) was noticed wi th an increase in BAP (1 to 4 mg/l). Whereas at higher concentrations of BA P (5 to 6 mg/l), there was a decline in shoot initiation (57 to 50%). Regen erants with 1 to 3 cms in height were transferred to rooting media and 78% rooting was observed on MS media supplemented with 4 mg/l IAA + 0.5 mg/l KN . The rooted plants were transferred to soil and showed 100% survival rate.