T. Matsumoto et al., Transient over-expression of NGFI-A gene suppresses NGF-induced neurite outgrowth in PC12 cells, NEUROREPORT, 11(5), 2000, pp. 1001-1005
Using an inducible gene expression system (Tet-ON system), the role of NGFI
-A gene during the neuronal differentiation of PC12 cells was examined. Whe
n NGFI-A was transiently overexpressed, no obvious effects on cell prolifer
ation or neurite outgrowth were observed. Interestingly, however, NGFI-A ov
er-expression resulted in significant retardation in NGF-induced neurite ou
tgrowth. Similar suppressive effects were observed also on the v-K-ras-indu
ced neurite outgrowth. These results raise the possibility that NGFI-A prot
ein may play some negative role in NGF signaling. NeuroReport 11:1001-1005
(C) 2000 Lippincott Williams & Wilkins.