This study investigated the relationship between external and middle ear fa
ctors and hearing screening results by automated auditory brain stem respon
se (ABR) and transient-evoked otoacoustic emissions (EOAEs). The ears of 20
0 healthy newborns aged 5 to 48 hours underwent screening by ABR and EOAE,
followed by otoscopic examination. The pass rates for ABR and EOAE were 91%
and 58.5%, respectively. On otoscopic examination, 28% (112/400) ears had
occluding vernix obscuring the view of the tympanic membrane. Cleaning of v
ernix was successfully performed in all but 2 ears that had occluding verni
x. Cleaning of vernix significantly increased the pass rates of all 400 ear
s for ABR and EOAE to 96% and 69%. Decreased tympanic membrane mobility was
found in 22.7% (90/396) of ears that were evaluated otoscopically. Decreas
ed tympanic membrane mobility had a significant effect on EOAE screening; o
nly 33.4% of ears passed EOAE testing. Decreased tympanic membrane mobility
did not significantly affect pass rates for ABR screening; 95% of these ea
rs passed the automated ABR screen, implications for newborn hearing screen
ing are discussed.