GroEL single-ring solution structure obtained by small-angle neutron scattering

Authors
Holzinger, J Heumann, H Manakova, E Rossle, M Vanatalu, K Wiedenmann, A May, RP
Citation
J. Holzinger et al., GroEL single-ring solution structure obtained by small-angle neutron scattering, PHYSICA B, 276, 2000, pp. 528-529
Citations number
5
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
PHYSICA B
ISSN journal
09214526 → ACNP
Volume
276
Year of publication
2000
Pages
528 - 529
Database
ISI
SICI code
0921-4526(200003)276:<528:GSSSOB>2.0.ZU;2-5
Abstract
In order to study the importance of interaction between the two rings that form the GroEL cylinder, we investigated a heptameric GroEL mutant (single ring, SR) by small-angle neutron scattering. We measured the scattering cur ves of single rings and of complexes of single rings with GroES and ADP in D2O. Preliminary model calculations revealed relevant differences between t he model based on the GroEL X-ray crystal structure and the experimental da ta. (C) 2000 Elsevier Science B.V. All rights reserved.