LOW SPECIFICITY OF PGP9.5 EXPRESSION FOR DETECTION OF MICROMETASTATICNEUROBLASTOMA

To determine the specificity of neuroendocrine protein gene product (P GP9.5) gene transcripts for detecting micrometastatic neuroblastoma, w e have used a highly sensitive polymerase chain reaction (PCR) techniq ue to evaluate expression of this gene in normal blood and bone marrow . While expression of the tyrosine hydroxylase gene was not detected i n any normal sample, low-level PGP9.5 expression was detected in eight out of ten blood and seven of 12 marrow samples. PGP9.5 gene transcri pts in normal tissues have the potential to interfere with the detecti on of micrometastatic neuroblastoma.