Optimization of the room-temperature phosphorescence of the 6-bromo-2-naphthol-alpha-cyclodextrin system in aqueous solution

The influence of different factors on the room-temperature phosphorescence (RTP) emission from the inclusion complex between alpha-cyclodextrin (alpha -CD) and 6-bromo-2-naphthol (BN) was analyzed. Although RTP signals are det ected even in aerated solutions, an efficient enhancement of the phosphores cence emission (about 13 times) is obtained when the solutions of the compl ex are deaerated with nitrogen bubbling, while quenching is produced when s odium sulfite is used for deoxygenation. Association constants of the 1:1 a nd 2:1 alpha-CD-BN complexes have been evaluated by molecular absorption an d fluorimetric methods. Exciting at 287 nm, the RTP phosphorescence emissio n showed two maxima located at 500 and 535 nm and a shoulder at 577 nm. The RTP emission increases with the irradiation time of the sample by the xeno n lamp of the fluorimeter, until it achieves a constant value after around 10 min of irradiation. The addition of organic molecules such as alcohols a nd bromoalcohols as a third component of the system produces an enhancement of the RTP emission smaller than that obtained in the absence of them. The calibration graph for BN was linear for the range of concentrations betwee n 0.4 and 2.0 mu g ml(-1) with a detection limit of 0.26 mu g ml(-1), with relative standard deviation (RSD) (n = 6) of 4%, for 1.6 mu g ml(-1). The s olution was transparent, and there was no precipitation. (C) 2000 Elsevier Science B.V. All rights reserved.