Canine serum protein patterns using high-resolution electrophoresis (HRE)

Serum protein values were determined in 26 healthy dogs using agarose gel e lectrophoresis (SPE), splitting the electrophoretic separation into six reg ions: albumin, alpha(1), alpha(2), beta(1), beta(2) and gamma-globulins. Hi gh-resolution electrophoresis (HRE) was used to separate single proteins. S erum proteins from dogs (26 healthy and 20 affected by various diseases) we re then characterized by electrophoretic immunofixation (IFE) and Sudan bla ck staining on HRE film. Haemoglobin and normal canine plasma and serum wer e used to identify haptoglobin and fibrinogen, respectively. In the standard pattern, determined by HRE, the following proteins were ide ntified: albumin, alpha(1)-lipoprotein (alpha(1)-region), haptoglobin and a lpha(2)-macroglobulin (alpha(2)-region), beta-lipoprotein and C3 (beta(1)-r egion), transferrin and IgM (beta(2)-region), IgG (mostly in gamma- region and partly in beta(2)-region). The HRE pattern shown by healthy dogs could be compared with those of dogs affected by various diseases to obtain clini cal information. (C) 2000 Harcourt Publishers Ltd.