Ej. Esselman et al., RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae : Lactuceae), AM J BOTANY, 87(4), 2000, pp. 591-596
Random Amplified Polymorphic DNA (RAPD) markers were used to measure geneti
c diversity within and divergence among species of Dendroseris (Asteraceae:
Lactuceae), a genus endemic to the Juan Fernandez Islands, Chile. Results
were compared to previous studies employing allozymes. For five of the spec
ies. RAPD banding patterns distinguished all individuals examined, and diff
erent mutilocus genotypes were found even in species exhibiting no allozyme
diversity. RAPD band diversities ranged from 0.003 to 0.022 within species
; >90% of total diversity was among species and <10% within them. Relative
levels of allozyme and RAPD diversity were similar for some species, partic
ularly those with highest and lowest diversities, but overall there was no
significant correlation. Relationships inferred from a neighbor-joining tre
e generated from RAPD bands were similar to results obtained from morpholog
y, chloroplast DNA (cpDNA) restriction site mutations, and sequences from t
he internal transcribed spacer regions of nuclear ribosomal DNA (ITS), but
somewhat better resolution was achieved. Relationships shown by allozymes d
iffered from trees based on other data; this ostensibly is a result of the
sharing of ancestral alleles and the absence of alleles generated subsequen
t to speciation. Dendroseris represents an example where RAPD markers, beca
use of their greater variability, provide a useful alternative to allozymes
for assessing diversity in rare species endemic to oceanic islands and for
resolving relationships among the species.