A histologic and histomorphometric evaluation of pulpal reactions following rapid palatal expansion

The aim of this study was to investigate the effects of the heavy forces ex erted by the rapid palatal expansion (RPE) appliance on the pulpal tissue o f anchor premolar teeth and to evaluate these effects by histologic and his tomorphometric methods. Materials consisted of 34 sound upper premolars, ex tractions of which were required as part of orthodontic treatment. Twenty-t hree teeth were extracted after RPE; the remaining 11 teeth, which had not undergone any orthodontic force, were taken as controls. After extraction, the teeth were prepared for histologic examination under light microscopy. Histomorphometric measurements were performed by using image analysis. The data gained were statistically evaluated by nonparametric tests. The null h ypothesis was that there was no difference between pulpal tissues of contro l and test teeth with respect to different durations of RPE. Our results re veal that the null hypothesis was rejected. Of the parameters evaluated, ve ssel area and minimum and maximum vessel diameters showed significant diffe rences among the groups. Especially the difference between the control and 3-month groups had marked statistical significance. In conclusion, forces a pplied by RPE appliances caused an adaptive vascular tissue response, as we ll as fibrotic changes, in the "affected" upper premolars.