Amperometric enzyme immunosensor for the determination of the antigen of the pathogenic fungi Trichophyton rubrum

An amperometric enzyme immunosensor for the determination of antigen (Ag) o f pathogenic fungi Trichophyton rubrum (TR) was developed. The immunosensor is based on a specific immunoreaction of Ag with antibodies which were imm obilized together with cholinesterase into a nitrocellulose membrane (biose nsitive part of immunosensor). The biosensitive part could be regenerated a nd is stable for at least 35 days. The developed immunosensor allows the de termination of down to 1 x 10(-15) mg ml(-1) of Ag TR. The total time of im munoassay is less than 20 min. The method was applied for the determination of Ag TR in human blood serum. (C) 2000 Elsevier Science B.V. All rights r eserved.