A high-throughput screening method for the determination of aqueous drug solubility using laser nephelometry in microtiter plates

The determination of aqueous solubility in a high-throughput screening envi ronment is invaluable in the selection of the most promising potential drug candidates. We describe a fast method based on laser nephelometry that can determine the solubility of potential drug candidates (usually from combin atorial chemistry) supplied as dimethyl sulfoxide (DMSO) solutions in 96-we ll plates. In the sample, the percent DMSO is kept constant allowing direct comparison of results. The nephelometric method has been shown to produce results equivalent to those produced by an HPLC method and to be largely un affected by colored solutions.