Identification of regulatory regions of the putative tumor suppressor geneDMBT1

The lack of expression of the putative tumor suppressor gene DMBT1 has been described in gliomas, lung carcinomas, and other malignancies. To investig ate the mechanisms regulating DMBT1 expression rye have screened a human ge nomic library with a 5' cDNA probe and identified a fragment of approximate ly 3 kb upstream of the proposed coding sequence of DMBT1. This fragment co ntains subregions that may up- or down-regulate the expression of a reporte r gene. These findings may help to elucidate the mechanisms regulating the alternative splicing and the tissue specific expression of DMBTI. (C) 2000 Academic Press.