Live oral poliovirus vaccines do not contain detectable simian virus 40 (SV40) DNA

Authors
Sierra-Honigmann, A Krause, PR
Citation
A. Sierra-honigmann et Pr. Krause, Live oral poliovirus vaccines do not contain detectable simian virus 40 (SV40) DNA, BIOLOGICALS, 28(1), 2000, pp. 1-4
Citations number
16
Categorie Soggetti
Microbiology
Journal title
BIOLOGICALS
ISSN journal
10451056 → ACNP
Volume
28
Issue
1
Year of publication
2000
Pages
1 - 4
Database
ISI
SICI code
1045-1056(200003)28:1<1:LOPVDN>2.0.ZU;2-Y
Abstract
Prior to 1962, poliovirus vaccines produced in rhesus monkey kidney cells w ere contaminated with SV40. Recent studies reporting the detection of SV40 in human tumours raised concern that SV40 may be oncogenic in humans. To pr ovide further assurance that currently used poliovirus vaccines are not con taminated with SV40, we used the polymerase chain reaction (PCR) to search for SV40 DNA in live oral poliovirus vaccines manufactured in the United St ates between 1972 and 1996. SV40 DNA sequences were not found in any of the vaccine lots tested.