Low frequency vibrations and structural characterization of a murine IgG2amonoclonal antibody studied by Raman and IR spectroscopies

Authors
Picquart, M Haro-Poniatowski, E Morhange, JF Jouanne, M Kanehisa, M
Citation
M. Picquart et al., Low frequency vibrations and structural characterization of a murine IgG2amonoclonal antibody studied by Raman and IR spectroscopies, BIOPOLYMERS, 53(4), 2000, pp. 342-349
Citations number
31
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOPOLYMERS
ISSN journal
00063525 → ACNP
Volume
53
Issue
4
Year of publication
2000
Pages
342 - 349
Database
ISI
SICI code
0006-3525(20000405)53:4<342:LFVASC>2.0.ZU;2-M
Abstract
The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the seco ndary structure is predominantly of the beta-sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 a,ld 94 cm(-1) was observed This band has been att ributed to vibrations of elastic body. The results are interpreted using a simple elastic model. (C) 2000 John Wiley & Sons, Inc.