Co-immobilization of manganese peroxidase from Phlebia radiata and glucoseoxidase from Aspergillus niger on porous silica beads

Authors
Van Aken, B Ledent, P Naveau, H Agathos, SN
Citation
B. Van Aken et al., Co-immobilization of manganese peroxidase from Phlebia radiata and glucoseoxidase from Aspergillus niger on porous silica beads, BIOTECH LET, 22(8), 2000, pp. 641-646
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
8
Year of publication
2000
Pages
641 - 646
Database
ISI
SICI code
0141-5492(200004)22:8<641:COMPFP>2.0.ZU;2-K
Abstract
Manganese peroxidase (MnP) from Phlebia radiata and glucose oxidase from As pergillus niger were co-immobilized on porous silica beads. Immobilization of both enzymes on the same carrier provided an integrated system in which H2O2 required by MnP was produced by glucose oxidase. The immobilization pr ocess resulted in a decrease of both enzymatic activities and substrate aff inities. However, immobilization improved the stability of MnP against H2O2 or high pH, as well as the storage stability of this enzyme.