Substrate-dependent production and some properties of a thermostable, alpha-galactosidase from Rhodothermus marinus

Authors
Blucher, A Karlsson, EN Holst, O
Citation
A. Blucher et al., Substrate-dependent production and some properties of a thermostable, alpha-galactosidase from Rhodothermus marinus, BIOTECH LET, 22(8), 2000, pp. 663-669
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
8
Year of publication
2000
Pages
663 - 669
Database
ISI
SICI code
0141-5492(200004)22:8<663:SPASPO>2.0.ZU;2-Q
Abstract
alpha-Galactosidase activity in Rhodothermus marinus is dependent on the co mposition of the growth media. A maximum of 46 mu U g(-1) cell dry weight w as obtained using minimal medium with galactooligo- or polysaccharides as s ingle carbon source. An enzyme hydrolysing both high and low molecular weig ht galacto-saccharides was partly purified from the cell fractions. The mol ecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally a ctive at 85 degrees C, with a half-life of 2 h at 75 degrees C, and had a b road pH range (4-8).