A simple method to separate cellulose-binding domains of fungal cellulasesafter digestion by a protease

Core-binding domains of fungal cellulases from Trichoderma reesei were puri fied using a new and simple technique. Cellulases were hydrolysed with papa in and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capil lary electrophoresis. This methodology produced a yield of 85% of binding d omains.