TEA1 AND THE MICROTUBULAR CYTOSKELETON ARE IMPORTANT FOR GENERATING GLOBAL SPATIAL ORDER WITHIN THE FISSION YEAST-CELL

Fission yeast cells identify and maintain growing regions exactly oppo sed at the ends of a cylindrical cell. tea1 mutants disrupt this organ ization, producing bent and T-shaped cells. We have cloned teal and sh own that teal is located at the cell poles. Microtubules are continuou sly required to transfer teal to the cell ends, and teal is located at the ends of microtubules growing toward the cell poles. We suggest th at teal acts as an end marker, directing the growth machinery to the c ell poles. tea1 is down-regulated in cells treated with pheromone that grow toward a mating partner and no longer maintain their ends exactl y opposed. teal may also influence microtubular organization, affectin g the maintenance of a single central axis.