In metazoa, assembly of spliceosomal U snRNPs requires nuclear export of U
snRNA precursors. Export depends upon the RNA cap structure, nuclear cap-bi
nding complex (CBC), the export receptor CRM1/Xpo1, and RanGTP. These compo
nents are however insufficient to support U snRNA export. We identify PHAX
(phosphorylated adaptor for RNA export) as the additional factor required f
or U snRNA export complex assembly in vitro. In vivo, PHAX is required for
U snRNA export but not for CRM1-mediated export in general. PHAX is phospho
rylated in the nucleus and then exported with RNA to the cytoplasm, where i
t is dephosphorylated. PHAX phosphorylation is essential for export complex
assembly while its dephosphorylation causes export complex disassembly. Th
e compartmentalized PHAX phosphorylation cycle can contribute to the direct
ionality of export.