Evaluation of the von Willebrand factor antigen (vWF-Ag) assay using an immuno-turbidimetric method (STA Liatest (R) vWF) automated on the MDA (R)-180 coagulometer
K. Sukhu et al., Evaluation of the von Willebrand factor antigen (vWF-Ag) assay using an immuno-turbidimetric method (STA Liatest (R) vWF) automated on the MDA (R)-180 coagulometer, CLIN LAB H, 22(1), 2000, pp. 29-32
The standard enzyme-linked immunosorbent assay (ELISA) test for von Willebr
and factor antigen (vWF-Ag), though sensitive and specific, does not delive
r the flexibility to handle single sample assessments economically or provi
de rapid, emergency testing capability. The present study examined the perf
ormance characteristics of an immunoturbidimetric assay kit (STA Liatest(R)
) modified for automation on the MDA(R)180 coagulation analyser using a sup
plied protocol, One hundred and sixteen patient samples were assessed by bo
th the standard and the modified method. The correlation coefficient was 0.
98 across the range of values 1-487 IU/dl, Above 200 IU/dl, where specimen
dilution was indicated, there was greater diversity (r = 0.86) between the
techniques. Plotting the difference between methods against the mean of bot
h showed excellent agreement between methods below 100 IU/dl vWF. The intra
-assay and interassay coefficients of variation were less than 3% at both l
ow and normal range levels. The MDA(R)180 automated vWF assay merits consid
eration as an alternative to ELISA testing that provides random access and
result availabilty within 30 min.