A novel multi-purpose cassette for repeated integrative epitope tagging ofgenes in Saccharomyces cerevisiae

Authors
De Antoni, A Gallwitz, D
Citation
A. De Antoni et D. Gallwitz, A novel multi-purpose cassette for repeated integrative epitope tagging ofgenes in Saccharomyces cerevisiae, GENE, 246(1-2), 2000, pp. 179-185
Citations number
11
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
03781119 → ACNP
Volume
246
Issue
1-2
Year of publication
2000
Pages
179 - 185
Database
ISI
SICI code
0378-1119(20000404)246:1-2<179:ANMCFR>2.0.ZU;2-1
Abstract
Gene tagging can be achieved by homologous recombination in yeast. The kan( t) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules contai ning a tag-loxP-kanMX-loxP cassette are described that allow tagging of dif ferent genes by using the kan(r) marker repeatedly. (C) 2000 Elsevier Scien ce B.V. All rights reserved.