Fission yeast (Schizosaccharomyces pombe) centromeres are composed of large
(40-100 kb) inverted repeats that display heterochromatic features, thus p
roviding a good model for higher eukaryotic centromeres. The association of
three proteins that mediate region-specific silencing across centromere 1
has been mapped by quantitative chromatin immunoprecipitation. Swi6 and Chp
1 are confined to the flanking outer repeats and Swi6 can spread across at
least 3 kb of extraneous chromatin in cen1. In contrast, Mis6 coats the inn
er repeats and central core, tRNA genes demarcate this transition zone. The
se analyses clearly define two distinct domains within this complex centrom
ere which interact with different proteins.