Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks

Amplified fragment length polymorphism (AFLP) markers with a total of 256 E coRI + ANN- MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 f ragments per primer combination were amplified, ranging from a maximum of 2 26 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By us ing 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AF LP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and ran domly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both h omoeologous and non-homoeologous groups, respectively, whereas additional f ragments were detected in N1BT1A, N5AT5D, and N6BT6A lines.