Chromosomal location of a Triticum timopheevii - derived powdery mildew resistance gene transferred to common wheat

A dominant powdery mildew resistance gene introduced from Triticum timophee vii in line 146-155-T of common wheat, Triticum aestivum, was located on ch romosome 6B by monosomic analysis. Restriction fragment length polymorphism (RFLP) and microsatellite analyses detected the presence of a T. timopheev ii segment, translocated to chromosome 6B, with breakpoints between the loc i Xpsr8/Xpsr964 on 6BS and Xpsr154/Xpsr546 on 6BL. The novel powdery mildew resistance gene, which has been designated Pm27, was shown to cosegregate with the microsatellite locus Xpsp3131, which is located on the introgresse d T. timopheevii segment. The molecular data confirm the location of Pm27 o n the translocated 6B chromosome.