Conventional methods for the detection of cholera toxin (CT) production by
vibrios are not readily available to most laboratories. A modification is d
escribed here of a simplified method standardised earlier and based on the
degradation of nicotinamide adenine dinucleotide (NAD) by CT; this is simpl
e and can be carried out in small laboratories also. It is also easy to per
form, and gives reproducible results.