The structure of short dough and biscuit has been characterized at a macros
copic level (dimensions, bulk structure) and a microscopic level (starch da
mage, protein aggregates, microstructure) by physical and biochemical metho
ds. The baking process of short dough induces a large decrease of the produ
ct bulk density from 1.26 to 0.42 (+/-0.01) g.cm(-3) for final biscuit, lea
ding to a cellular solid with a thin colored surface and a porous inner str
ucture. Proteins appear aggregated in biscuit when compared to short dough,
whereas starch granules remain almost intact in biscuits. The components wh
ich are involved in the cohesiveness of short dough and biscuit final struc
ture have been identified. They suggest that short dough is a suspension of
solid particles in a liquid phase being an emulsion of lipids in a concent
rated sugar solution. The role of sugars in biscuit, structure suggest that
biscuit structure is a composite matrix of protein aggregates, lipids and
sugars, embedding starch granules.