Heterologous expression of bacterial epoxyalkane : Coenzyme M transferase and inducible coenzyme M biosynthesis in Xanthobacter strain Py2 and Rhodococcus rhodochrous B276

Coenzyme M (CoM) (2-mercaptoethanesulfonic acid) biosynthesis is shown to b e coordinately regulated with the expression of the enzymes of alkene and e poxide metabolism in the propylene-oxidizing bacteria Xanthobacter strain P y2 and Rhodococcus rhodochrous strain B276. These results provide the first evidence for the involvement of CoM in propylene metabolism by R. rhodochr ous and demonstrate for the first time the inducible nature of eubacterial CoM biosynthesis.