Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract

Spinal and bulbar muscular atrophy (SBMA) is one of a group of human inheri ted neurodegenerative diseases caused by polyglutamine expansion. We have p reviously demonstrated that the SBMA gene product, the androgen receptor pr otein, is toxic and aggregates when truncated. Heat shock proteins function as molecular chaperones, which recognize and renaturate misfolded protein (aggregate). We thus assessed the effect of a variety of chaperones in a cu ltured neuronal cell model of SBMA. Overexpression of chaperones reduces ag gregate formation and suppresses apoptosis in a cultured neuronal cell mode l of SBMA to differing degrees depending on the chaperones and their combin ations. Combination of Hsp70 and Hsp40 was the most effective among the cha perones in reducing aggregate formation and providing cellular protection, reflecting that Hsp70 and Hsp40 act together in chaperoning mutant and disa bled proteins. Although Hdj2/Hsdj chaperone has been previously reported to suppress expanded polyglutamine tract-formed aggregate, Hsdj/Hdj2 showed l ittle Effect in our system. These findings indicate that chaperones may be one of the key factors in the developing of CAG repeat disease and suggeste d that increasing expression level or enhancing the function of chaperones will provide an avenue for the treatment of CAG repeat disease.