Desensitisation of calcitonin gene-related peptide responsiveness but not adrenomedullin responsiveness in vascular smooth muscle cells

Adrenomedullin (ADM) and calcitonin gene-related peptide (CGRP) are distant ly related peptides. Both act through G protein-coupled receptors on vascul ar smooth muscle cells to increase-intracellular cAMP concentrations, causi ng vasorelaxation. Recent evidence suggests that both peptides bind to a co mmon heptahelical receptor, with specificity for each peptide being determi ned by a receptor activity modifying protein (RAMP). This hypothesis predic ts that each peptide should desensitise the cellular response to subsequent stimulation by the other. We have studied the patterns of desensitisation of ADM/CGRP receptors in rat aortic vascular smooth muscle cells. Cells wer e incubated for 20 min in either serum free medium (SFM), alone (control) o r in SFM containing vasoactive agonist (e.g. ADM 10(-8) M, CGRP 10(-7) M, a ngotensin II 10(-9) M or isoproterenol 10(-6)M). Cells were then washed and incubated for a further 20min in SFM containing a second agonist and 1 mM isobutyryl methyl xanthine. Cells were harvested and assayed for cAMP. Pre- exposure of cells to CGRP, isoproterenol, angiotensin II or ADM, decreased cAMP generation in response to subsequent stimulation with CGRP by 84% (+/- 5), 66% (+/-18), 45% (+/-5) and 60% (+/-10) respectively (mean +/- s.D.). Pre-incubation of cells with 100 nM H-89, a protein kinase A (PKA) inhibito r, abolished the desensitisation of CGRP by itself, implying that this dese nsitisation was mediated through PKA. In contrast, there was no attenuation of the cAMP response to stimulation with ADM by pre-exposure to ADM and al l other agonists tested. Identical results were seen with or without PKA in hibition by H-89. These results indicate that the ADM receptor does not des ensitise over this time period in RAVSMCs, in contrast to the CGRP receptor , which is desensitised by pre-exposure to CGRP and other vasoactive agonis ts. These data also suggest that ADM and CGRP act through separate receptor s in these cells.