A new theory for the molecular mechanism of sludge granulation, namely prot
on translocation-dehydration theory, was proposed in this study. Based on t
he consideration of biological activity of the bacterial cells, the bacteri
al surface dehydration caused by proton translocating activity was suggeste
d to initiate the sludge granulation. The overall granulation process in th
is new theory included four stages: dehydration of bacterial surfaces, embr
yonic granule formation, granule maturation, and postmaturation. Some pheno
mena involved in practical sludge granulation were adequately explained by
this new theory.