Bioactive extracts from wild blueberries were isolated, fractionated, and a
nalyzed, Antioxidant activity, cardioprotective capacity, and ability to in
hibit the initiation stage of chemically-induced carcinogenesis were evalua
ted. Many fractions had antioxidant activity, especially those rich in anth
ocyanins and proanthocyanidins. The ease of isolation of bioactive compound
s and the ability to obtain accurate bioassays depended strongly on the sou
rce material used. Extracts from spray-dried formulations containing magnes
ium citrate and tri-calcium phosphate provided easier materials for bioacti
vity assessment and demonstrated antioxidant, cancer preventive and cardiop
rotective activity, Extracts with potent antioxidant activity degraded read
ily, underscoring the need for rapid alternative fractionation tactics and
rigorously controlled storage conditions.