ANALYSIS OF PHOSPHOFRUCTOKINASE SUBUNITS AND ISOZYMES IN ASCITES TUMOR-CELLS AND ITS ORIGINAL TISSUE, MURINE MAMMARY-GLAND

Phosphofructokinase (PFK) subunits and isozymes have been examined in ascites tumor cells and murine mammary gland, the tissue from where th is tumor originated, Ascites tumor was found to contain predominantly the C-type subunit, whereas the L-type subunit was more abundant in ma mmary gland, An altered M-type subunit of lower electrophoretic mobili ty was found in both cell types and no M-4 homotetramers were detected in either of them, Characteristic regulatory properties of ascites tu mor PFK, i.e. insensitivity to fructose-1,6-P-2 activation and inhibit ion by P-enolpyruvate, were also observed in the mammary gland isozyme , The nature of these properties and the contribution of the distinct subunit types to fructose-1,6-P-2 activation are discussed. (C) 1997 F ederation of European Biochemical Societies.