RH maps of bovine chromosomes 15 and 29: conservation of human chromosomes11 and 5

Comparative mapping data on evolutionary conserved coding sequences and syn teny maps between human and cattle are insufficient to define the extent an d distribution of conserved segments between these two species, because the order of loci is often rearranged. A 5000-rad cattle whole-genome radiatio n hybrid (WG-RH) panel was constructed to provide high-resolution comparati ve maps and also to integrate linkage maps of microsatellites with evolutio nary conserved genes and transcripts in a single ordered map. We used the W G-RH panel to construct radiation hybrid maps of bovine Chromosomes (Chrs) 15 and 29 (BTA15 and BTA29), integrating microsatellites from published lin kage maps with selected genes. The comprehensive map of BTA15 consists of 2 4 markers. 13 of which were placed in the framework map. Eleven molecular m arkers compose the comprehensive map of BTA29. seven of which were placed i n the framework map. We identified the homologous regions between bovine Ch r 15 (BTA15) and human Chrs 5 and 11 (HSA5 and HSA11), as well as between B TA29 and HSA11, The present study demonstrates that WG-RH mapping is an eff icient method for integrating multiple genetic maps into one map and for in corporating monomorphic Type I loci into ordered maps for comparison betwee n species.