RT-PCR as a tool for systematic transcriptional analysis of large regions of the Bacillus subtilis genome

Authors
Hernandez, A Figueroa, A Rivas, LA Parro, V Mellado, RP
Citation
A. Hernandez et al., RT-PCR as a tool for systematic transcriptional analysis of large regions of the Bacillus subtilis genome, MICROBIO-UK, 146, 2000, pp. 823-828
Citations number
15
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY-UK
ISSN journal
13500872 → ACNP
Volume
146
Year of publication
2000
Part
4
Pages
823 - 828
Database
ISI
SICI code
1350-0872(200004)146:<823:RAATFS>2.0.ZU;2-4
Abstract
Transcriptional analysis of five different regions of the Bacillus subtilis 168 genome, comprising a total of 175 kb encoding newly identified genes, was carried out using the RT-PCR technique as part of the functional analys is of whole genome of this bacterium, Amplification of mRNA fragments allow ed the detection of both highly and poorly transcribed genes covering 81% o f putative ORFs, and also the monitoring of variations in the expression le vel among genes differentially expressed during particular bacterial growth phases.